European Journal of Chemistry

Pure component contribution (PCCA) and synergy interval partial least squares (siPLS) algorithms for efficient resolution and quantification of overlapped signals; an application to novel antiviral tablets of daclatasvir, sofosbuvir and ribavirin


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Maha Mahmoud Abou El-Alamin
Maha Abd Elrahman Sultan
Maha Hegazy
Alastair William Wark
Marwa Mohamed Azab


Daclatasvir (DAC), sofosbuvir (SOF) and ribavirin (RIB) have been recently co-formulated in tablet dosage form for the treatment of Hepatitis C virus infections. In this work, the resolution and quantitation of overlapped spectral signals was achieved by both univariate and multivariate algorithms. Pure component contribution algorithm (PCCA) as a novel approach was applied along with factor based partial least squares (PLS) algorithms using both full range and synergistic intervals (siPLS). Each drug could be determined at its λmax using PCCA, while PLS and siPLS were used for multivariate determination of the three components. Good linear relationships were obtained in the ranges of 5.45-16.35, 4.40-44.00 and 5.50-35.00 µg/mL for DAC, SOF and RIB, respectively, by PCCA. The PLS and siPLS models were built for the three compounds each in the concentration range of 2.00-10.00, 10.00-20.00 and 10.00-26.00 µg/mLfor DAC, SOF and RIB, respectively. Validation of the proposed methods was ascertained according to ICH guidelines for PCCA and through the use of internal and external validation sets for PLS and SiPLS models. The three methods were successfully applied for determination of DAC, SOF and RIB in pure form and in tablets.

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How to Cite
El-Alamin, M. M. A.; Sultan, M. A. E.; Hegazy, M.; Wark, A. W.; Azab, M. M. Pure Component Contribution (PCCA) and Synergy Interval Partial Least Squares (siPLS) Algorithms for Efficient Resolution and Quantification of Overlapped Signals; An Application to Novel Antiviral Tablets of Daclatasvir, Sofosbuvir and Ribavirin. Eur. J. Chem. 2019, 10, 350-357.

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[1]. Alter, M. J. World J. Gastroentero. 2007, 13(17), 2436-2441.

[2]. Pelosi, L. A.; Voss, S.; Liu, M.; Gao, M.; Lemm, J. A. Antimicrob. Agents Ch. 2012, 56(10), 5230-5239.

[3]. Alavian, S. M.; Rezaee-Zavareh, M. S. Hepat. Mon. 2016, 16(9), e41077, 1-15.

[4]. Guedj, J.; Dahari, H.; Rong, L.; Sansone, N. D.; Nettles, R. E.; Cotler, S. J.; Layden, T. J.; Uprichard, S. L.; Perelson, A. S. Proc. Natl. Acad. Sci. USA 2013, 110(10), 3991-3996.

[5]. Sultan, M. A.; El-Alamin, M. M. A.; Wark, A. W.; Azab, M. M. Spectrochim. Acta, A 2019, 211, 52-58.

[6]. Sulkowski, M. S.; Gardiner, D. F.; Rodriguez-Torres, M.; Reddy, K. R.; Hassanein, T.; Jacobson, I.; Lawitz, E.; Lok, A. S.; Hinestrosa, F.; Thuluvath, P. J. New Engl. J. Med. 2014, 370(3), 211-221.

[7]. Sweetman, S. C.; Blake, P., Martindale, 37th edition. Pharmaceutical Press, 2011.

[8]. Fontaine, H.; Hezode, C.; Zoulim, F.; Samuel, D.; Bourliere, M.; Haour, G.; Dorival-Mouly, C.; Leroy, V.; De Ledinghen, V.; Lucier, S. The international Liver Congress™ 2015-50th Annual meeting of the European Association for the Study of the Liver, 2015, p. S278.

[9]. Pol, S.; Corouge, M.; Vallet-Pichard, A. Hepat Med. 2016, 8, 21-26.

[10]. Magdy, A. W.; Samia, M. M.; Sobhy, M. E. A.; Mohamed, S. E. J. Pharm. Biol. Sci. 2017, 12 (5), 60-68.

[11]. Hassan, W. S.; Elmasry, M. S.; Elsayed, H. M.; Zidan, D. W. Spectrochim. Acta A 2018, 202, 159-173.

[12]. B.M. Wise, N. B. G., Version 2.1.1 Ed. Eigenvector Research, Inc.,. 2001.

[13]. MATLAB 6.5, The MathWorks, Inc., Natick, Massachusetts, United States.

[14]. Brereton, R. G. Analyst 1997, 122(12), 1521-1529.

[15]. Hegazy, M. A. M. Spectrochim. Acta A 2015, 151, 405-414.

[16]. Toubar, S. S.; Hegazy, M. A.; Elshahed, M. S.; Helmy, M. I. Spectrochim. Acta A 2016, 163, 89-95.

[17]. Haaland, D. M.; Thomas, E. V. Anal. Chem. 1988, 60(11), 1193-1202.

[18]. Hegazy, M. A.; Boltia, S. A.; Fayed, A. S.; Musaed, A. Spectrochim. Acta A 2018, 202, 359-367.

[19]. Parisotto, G.; Ferrao, M. F.; Müller, A. L.; Müller, E. I.; Santos, M. F.; Guimaraes, R. C.; Dias, J. C.; Flores, E. M. Energ Fuel 2010, 24(10), 5474-5478.

[20]. Shah, D.; He, Q. P.; Wang, J. IFAC PapersOnLine 2018, 51(18), 369-374.

[21]. Guideline, I. H. T. International Conference on Harmonization, Geneva, Switzerland, 2005; pp. 11-12.

[22]. Elfatatry, H. M.; Mabrouk, M. M.; Hammad, S. F.; Mansour, F. R.; Kamal, A. H.; Alahmad, S. J. AOAC Int. 2016, 99(5), 1247-1251.

[23]. Hemmateenejad, B.; Akhond, M.; Samari, F. Spectrochim. Acta A 2007, 67(3-4), 958-965.

[24]. El-Gindy, A.; Emara, S.; Shaaban, H. J. Pharmaceut. Biomed. 2007, 43(3), 973-982.

[25]. Hadad, G. M.; El-Gindy, A.; Mahmoud, W. M. Spectrochim. Acta A 2008, 70(3), 655-663.

[26]. Martens, H.; Naes, T., Multivariate calibration: A user-friendly guide to multivariate calibration and classification. John Wiley & Sons, New York: 1989.

[27]. Espinosa-Mansilla, A.; Duran-Meras, I.; Salinas, F. J. Pharmaceut. Biomed. 1998, 17(8), 1325-1334.

[28]. Wagieh, N. E.; Hegazy, M. A.; Abdelkawy, M.; Abdelaleem, E. A. Talanta 2010, 80(5), 2007-2015.

[29]. Miller, J.; Miller, J. C., Statistics and chemometrics for analytical chemistry. Pearson Education, Canada, 2005.

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