European Journal of Chemistry 2014, 5(1), 11-17. doi:10.5155/eurjchem.5.1.11-17.885

Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma


Mohamed Rizk (1) , Safaa Shafik Toubar (2) , Maha Mahmoud Abou El-Alamin (3,*) , Marwa Mohamed Mahmoud Azab (4)

(1) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(*) Corresponding Author

Received: 25 Jul 2013, Accepted: 13 Aug 2013, Published: 31 Mar 2014

Abstract


A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.


Keywords


Stability; Itraconazole; Anti-fungal drugs; Method validation; Micellar liquid chromatography; Optimization and system suitability

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DOI: 10.5155/eurjchem.5.1.11-17.885

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How to cite


Rizk, M.; Toubar, S.; El-Alamin, M.; Azab, M. Eur. J. Chem. 2014, 5(1), 11-17. doi:10.5155/eurjchem.5.1.11-17.885
Rizk, M.; Toubar, S.; El-Alamin, M.; Azab, M. Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma. Eur. J. Chem. 2014, 5(1), 11-17. doi:10.5155/eurjchem.5.1.11-17.885
Rizk, M., Toubar, S., El-Alamin, M., & Azab, M. (2014). Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma. European Journal of Chemistry, 5(1), 11-17. doi:10.5155/eurjchem.5.1.11-17.885
Rizk, Mohamed, Safaa Shafik Toubar, Maha Mahmoud Abou El-Alamin, & Marwa Mohamed Mahmoud Azab. "Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma." European Journal of Chemistry [Online], 5.1 (2014): 11-17. Web. 22 Nov. 2019
Rizk, Mohamed, Toubar, Safaa, El-Alamin, Maha, AND Azab, Marwa. "Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma" European Journal of Chemistry [Online], Volume 5 Number 1 (31 March 2014)

DOI Link: https://doi.org/10.5155/eurjchem.5.1.11-17.885

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