European Journal of Chemistry

Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma



Main Article Content

Mohamed Rizk
Safaa Shafik Toubar
Maha Mahmoud Abou El-Alamin
Marwa Mohamed Mahmoud Azab

Abstract

A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.


icon graph This Abstract was viewed 1858 times | icon graph Article PDF downloaded 776 times

How to Cite
(1)
Rizk, M.; Toubar, S. S.; El-Alamin, M. M. A.; Azab, M. M. M. Micellar High Performance Liquid Chromatographic Determination of Itraconazole in Bulk, Pharmaceutical Dosage Forms and Human Plasma. Eur. J. Chem. 2014, 5, 11-17.

Article Details

Share
Crossref - Scopus - Google - European PMC
References

[1]. Ghannoum, M. A.; Rice, L. B. Clin. Microbiol. Rev. 1999, 12, 501-517.

[2]. British Pharmacopoeia, British Pharmacopoeia Commission, The Stationery Office, London, UK, 2012.

[3]. European Pharmacopoeia, 5th ed. Supplements 1-5. Strasbourg, Council of Europe, 2005-2006.

[4]. Miura, M.; Takahashi, N.; Nara, M.; Fujishima, N.; Kagaya, H.; Kameoka, Y. Ann. Clin. Biochem. 2010, 47, 432-439.
http://dx.doi.org/10.1258/acb.2010.010029

[5]. Gagliardi, L.; De-Orsi, D.; Chimenti, P.; Porra, R.; Tonelli, D. Anal. Sci. 2003, 19, 1195-1197.
http://dx.doi.org/10.2116/analsci.19.1195

[6]. Ohkubo, T.; Osanai, T. Ann. Clin. Biochem. 2005, 42, 94-98.
http://dx.doi.org/10.1258/0004563053492711

[7]. Khoschsorur, G.; Fruehwirth, F.; Zelzer, S. Antimicrob. Agents Chemother. 2005, 49, 3569-3571.
http://dx.doi.org/10.1128/AAC.49.8.3569-3571.2005

[8]. Yoo, S. D.; Lee, S. H.; Kang, E.; Jun, H.; Jung, J. Y.; Park, J. W.; Lee, K. H. Drug Dev. Ind. Pharm. 2000, 26, 27-34.
http://dx.doi.org/10.1081/DDC-100100324

[9]. AlRawithi, S.; Hussein, R.; Al-Moshen, I.; Raines, D. Ther. Drug Monit. 2001, 23, 445-448.
http://dx.doi.org/10.1097/00007691-200108000-00021

[10]. Gordien, J.; Pigneux, A.; Vigouroux, S.; Tabrizi, R.; Accoceberry, I. J. Pharm. Biomed. Anal. 2009, 50, 932-938.
http://dx.doi.org/10.1016/j.jpba.2009.06.030

[11]. Jaruratanasirikul, S.; Sriwiriyajan, S. Eur. J. Clin. Pharmacol. 2007, 63, 451-456.
http://dx.doi.org/10.1007/s00228-007-0280-x

[12]. Kumar, V.; Wang, L.; Riebe, M.; Tung, H.; Prud-homme, R. Mol. Pharm. 2009, 6, 1118-1124.
http://dx.doi.org/10.1021/mp900002t

[13]. Bharathi, V.; Hotha, K.; Sagar, P. V.; Kumar, S. S.; Reddy, P. R.; Naidu, A.; Mullangi, R. J. Chromatogr. B 2008, 868, 70-76.
http://dx.doi.org/10.1016/j.jchromb.2008.04.029

[14]. Wook Choi, Y.; Nam, D.; Kang, K. H.; Wook Ha, K.; Han, I. H.; Chang, B. K.; Yoon, M.; Lee, J. Bull. Korean Chem. Soc. 2006, 27, 291-294.

[15]. Rhim, S. Y.; Park, J. H.; Park, Y. S.; Kim, D. S.; Lee, M. H; Shaw, L. M; Kang, J. S. Pharmazie 2009, 64, 71-75.

[16]. Yao, M.; Chen, L. S.; Srinivas, N. R. J. Chromatogr. B 2001, 752, 9-16.
http://dx.doi.org/10.1016/S0378-4347(00)00505-3

[17]. Castro-Puyana, M.; Crego, A. L.; Marina, M. L. Electrophoresis 2006, 27, 887-895.
http://dx.doi.org/10.1002/elps.200500347

[18]. Breadmore, M.; Prochazkova, A.; Theurillat, R.; Thormann, W. J. Chromatogr. A 2003, 1014, 570-578.
http://dx.doi.org/10.1016/S0021-9673(03)00573-9

[19]. Zhang, C. X.; Von-Heeren, F.; Thormann, W. Anal. Chem. 1995, 67, 2070-2077.
http://dx.doi.org/10.1021/ac00109a028

[20]. Crego, A. L.; Gomez, J.; Marina, M. L.; Lavandera, J. L. Electrophoresis 2001, 22, 2503-2511.
http://dx.doi.org/10.1002/1522-2683(200107)22:12<2503::AID-ELPS2503>3.0.CO;2-5

[21]. Radoslaw, J. E.; Krzek, J. Acta Pol. Pharm. 2009, 66, 19-23.

[22]. El-Enany, N.; El-Sherbiny, D.; Belal, F. J. Chin. Chem. Soc. 2007, 54, 375-382.

[23]. Armstrong, D.; Henry, S. J. Liq. Chromatogr. R. T. 1980, 3, 657-662.
http://dx.doi.org/10.1080/01483918008060181

[24]. Berthod, A. J. Chromatogr. A 1997, 780, 191-206.
http://dx.doi.org/10.1016/S0021-9673(97)00195-7

[25]. Validation of analytical procedure: Text and Methodology. International Conference on Harmonization Q2(R1), 2005. Accessed at: http://www.bioforum.org.il/Uploads/Editor/karen/q2_r1_step4.pdf

[26]. Miller, J. C.; Miller, J. N. Statistics and Chemometrics for Analytical Chemistry, 5th edition. Pearson Education Limited: Harlow, England, 2005, pp. 39-73, 107-149, 256.

[27]. The United States Pharmacopeia, 30th rev., and the National Formulary 25th ed. Rockville, MD: The United States Pharmacopeial Convention, 2007.

Supporting Agencies

Faculty of Pharmacy, Helwan University, Cairo, Egypt and Egyptian Research and Development Company, Egypt
Most read articles by the same author(s)

Most read articles by the same author(s)

TrendMD

Dimensions - Altmetric - scite_ - PlumX

Downloads and views

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...
License Terms

License Terms

by-nc

Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at https://www.eurjchem.com/index.php/eurjchem/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (https://www.eurjchem.com/index.php/eurjchem/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).