European Journal of Chemistry

Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma



Main Article Content

Mohamed Rizk
Safaa Shafik Toubar
Maha Mahmoud Abou El-Alamin
Marwa Mohamed Mahmoud Azab

Abstract

A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.


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Rizk, M.; Toubar, S. S.; El-Alamin, M. M. A.; Azab, M. M. M. Micellar High Performance Liquid Chromatographic Determination of Itraconazole in Bulk, Pharmaceutical Dosage Forms and Human Plasma. Eur. J. Chem. 2014, 5, 11-17.

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