European Journal of Chemistry

Micellar high performance liquid chromatographic determination of Itraconazole in bulk, pharmaceutical dosage forms and human plasma

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Mohamed Rizk
Safaa Shafik Toubar
Maha Mahmoud Abou El-Alamin
Marwa Mohamed Mahmoud Azab


A micellar liquid chromatographic method was developed for determination of Itraconazole in bulk, dosage form and human plasma using µBondapack cyano column and a mobile phase consisting of 0.1 M sodium dodecyl sulphate, 20% 1-propanol, 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3.5). The UV detection was achieved at 258 nm. Various chromatographic parameters were studied, e.g. types of columns, pH of mobile phase, concentration of sodium dodecyl sulphate, 1-propanol, triethylamine, etc .The method was linear over the concentration range 16.4-320.0 µg/mL with regression coefficient 0.999 and limit of detection 5.4 µg/mL in bulk. Inter and intra-day results showed %RSD < 1.49%. The result obtained by the proposed method was compared with that obtained by the reference HPLC technique. Furthermore, the proposed method was successfully applied as stability-indicating method for determination of Itraconazole under different stressed conditions. The method showed good selectivity, repeatability, linearity and sensitivity according to the evaluation of the validation parameters.

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Rizk, M.; Toubar, S. S.; El-Alamin, M. M. A.; Azab, M. M. M. Micellar High Performance Liquid Chromatographic Determination of Itraconazole in Bulk, Pharmaceutical Dosage Forms and Human Plasma. Eur. J. Chem. 2014, 5, 11-17.

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