Development and validation of second derivative and synchronous spectrofluorimetric methods for determination of oxytocin and ergometrine maleate in their combined formulation
Mohamed Rizk (1) , Maha Mahmoud Abou El-Alamin (2,*) , Mervat Isaac Moawad (3)
(1) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, 11795, Cairo, Egypt
(*) Corresponding Author
Received: 23 Apr 2018, Accepted: 29 Jun 2018, Published: 30 Sep 2018
Simple and sensitive second derivative and synchronous spectrofluorimetric methods have been developed and validated for the quantitative determination of oxytocin and ergometrine maleate in their pure and combined dosage forms. The methods are based on the derivatization reaction of oxytocin with fluorescamine reagent, which yielded a highly fluorescent compound measured at 486 nm after excitation at 390 nm. Ergometrine was directly measured in combination with oxytocin, since it exhibits native fluorescence at 421 nm after excitation at 300 nm. Quantitation of oxytocin in presence of ergometrine was also successful at 482 and 477 nm using second derivative and synchronous spectrofluorimetry, respectively. Different experimental parameters were studied and optimized. The relative fluorescence intensity versus concentration plot was rectilinear over the range of 0.04-0.75 and 5-100 ng/mL for oxytocin and ergometrine, respectively. The methods were successfully applied for the determination of both drugs in their prepared combined ampoules. The methods were validated and compared with the reference chromatographic method; they revealed good accuracy and reproducible results. The proposed methods showed high accuracy and sensitivity, with no requirement of multiple steps or previous chemical separation.
This Abstract was viewed 272 times | PDF Article downloaded 86 times
. Nachtmann, F.; Krummen, K.; Maxl, F.; Riemer, E. Analytical Profiles of Drug Substances and Excipients, Edited by K. Florey, Academic Press, California, 1981, 10, 563-600.
. Sweetman, S. Martindale: The Complete Drug Reference. 37th Ed. The Pharmaceutical Press, London, 2011.
. The United States Pharmacopeia, 37, NF 32, vol. II, US Pharmacopeial Convention, Rockville, MD, 2014.
. The British Pharmacopeia, Vol. III, HMSO, London, 2015.
. Pask-Hughes, R. A.; Corran, P. H.; Calam, D. H. J. Chromatogr. A 1981, 214, 307-315.
. Pask-Hughes, R. A.; Hartley, R. E.; Gaines-Das, R. E. J. Biol. Stand. 1983, 11, 13-17.
. Walash, M. I.; Belal, F.; El-Enany, N.; El-Maghrabey, M. H. J. Lumin. 2011, 26, 342-348.
. Walash, M. I.; Belal, F.; El-Enany, N.; El-Maghrabey, M. H. J. Lumin. 2012, 27, 511-518.
. El-Enany, N.; Abdelal, A.; Belal, F. Chem. Cent. J. 2011, 5(56), 1-8.
. Rose, J. Advanced physico-chemical experiments, Pitman, London, 1964.
. Stein, S.; Bohlen, P.; Stone, J.; Dairman, W.; Undenfriend, S. Arch. Biochem. Biophys. 1973, 155, 203-212.
. Karpinska, J.; Sokol, A.; Skoczylas, M. Spectrochim. Acta A 2008, 71, 1562-1564.
. El-Enany, N. J. AOAC. Int. 2007, 90, 948-956.
. Belal, F.; Abdine, H.; Al-Majed, A. and Khalil, N. Y. J. Pharm. Biomed. Anal. 2002, 27, 253-260.
. Miller, J. N.; Miller, J. C. Statistics and Chemometrics for Analytical Chemistry, 5th Ed., Harlow, England, 2005, pp. 39-73, 107-149, 256.
. ICH Harmonized Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Q2(R1), Current Step 4 Version, Parent Guidelines on Methodology Dated November 6; 1996, incorporated in November, 2005.
How to cite
DOI Link: https://doi.org/10.5155/eurjchem.9.3.241-250.1724
- There are currently no refbacks.
Copyright (c) 2018 Authors
This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).
© Copyright 2019 • Atlanta Publishing House LLC • All Right Reserved.