European Journal of Chemistry 2012, 3(2), 152-155. doi:10.5155/eurjchem.3.2.152-155.570

Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin


Mohammad Abdul-Azim Mohammad (1) , Ehab Farouk Elkady (2) , Marwa Ahmed Fouad (3,*)

(1) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Umm Al-Qura University, Makkah Al-Mokarama, P.O. Box 715, Kingdom of Saudi Arabia
(2) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt
(3) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt
(*) Corresponding Author

Received: 13 Dec 2011, Accepted: 12 Jan 2012, Published: 30 Jun 2012

Abstract


A new, simple, accurate, and precise liquid chromatographic method has been developed and validated for the determination of two novel dipeptidylpeptidase-4 (DPP-4) inhibitors; namely vildagliptin (VLG) and saxagliptin HCl (SXG) simultaneously in their binary mixtures with metformin HCl (MET). Chromatographic separation was achieved on an Inertsil® CN-3 column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (4.6) - acetonitrile (15:85, v:v) at a flow rate of 1 mL/min with UV detection at 208 nm was performed. The liquid chromatographic method was used for the simultaneous determination of either VLG, SXG and MET in the range of 5-200, 0.5-20 and 50-2000 μg/mL, respectively. The methods developed were satisfactorily applied to the analysis of the pharmaceutical formulations and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.

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Keywords


Metformin; Saxagliptin; Vildagliptin; Isocratic elution; Pharmaceutical preparation; Reversed-phase liquid chromatography

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DOI: 10.5155/eurjchem.3.2.152-155.570

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Citations

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[1]. Shereen Mowaka, Dalia Mohamed
Novel contribution to the simultaneous analysis of certain hypoglycemic drugs in the presence of their impurities and degradation products utilizing UPLC-MS/MS
RSC Advances  5(74), 60467, 2015
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Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy  140, 229, 2015
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Stability-Indicating Liquid Chromatographic Method for Determination of Saxagliptin and Structure Elucidation of the Major Degradation Products Using LC-MS
Journal of Chromatographic Science  53(4), 554, 2015
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[5]. N. Singh, P. Bansal, M. Maithani, Y. Chauhan
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New Journal of Chemistry  42(4), 2459, 2018
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[6]. Aruna Gundala, KVSRG Prasad, Bharathi Koganti
Application of quality by design approach in RP-HPLC method development for simultaneous estimation of saxagliptin and dapagliflozin in tablet dosage form
Brazilian Journal of Pharmaceutical Sciences  55, , 2019
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[7]. Eman I. El-Kimary, Dalia A. Hamdy, Sara S. Mourad, Magda A. Barary
HPTLC Determination of Three Gliptins in Binary Mixtures with Metformin
Journal of Chromatographic Science  , bmv106, 2015
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[8]. Marwa S. Moneeb
Spectrophotometric and spectrofluorimetric methods for the determination of saxagliptin and vildagliptin in bulk and pharmaceutical preparations
Bulletin of Faculty of Pharmacy, Cairo University  51(2), 139, 2013
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[9]. Maha F. Abdel-Ghany, Omar Abdel-Aziz, Miriam F. Ayad, Mariam M. Tadros
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[10]. Hanan A. Merey, Nesrin K. Ramadan, Sherine S. Diab, Azza A. Moustafa
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Bulletin of Faculty of Pharmacy, Cairo University  55(2), 311, 2017
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[11]. Cristiane de Andrade, Graziela de Araújo Lock, Maiara Cássia Pigatto, Sandra Elisa Haas, Teresa Dalla Costa, Bibiana Verlindo de Araújo
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Biomedical Chromatography  28(12), 1722, 2014
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Journal of Analytical Chemistry  73(4), 303, 2018
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How to cite


Mohammad, M.; Elkady, E.; Fouad, M. Eur. J. Chem. 2012, 3(2), 152-155. doi:10.5155/eurjchem.3.2.152-155.570
Mohammad, M.; Elkady, E.; Fouad, M. Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin. Eur. J. Chem. 2012, 3(2), 152-155. doi:10.5155/eurjchem.3.2.152-155.570
Mohammad, M., Elkady, E., & Fouad, M. (2012). Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin. European Journal of Chemistry, 3(2), 152-155. doi:10.5155/eurjchem.3.2.152-155.570
Mohammad, Mohammad, Ehab Farouk Elkady, & Marwa Ahmed Fouad. "Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin." European Journal of Chemistry [Online], 3.2 (2012): 152-155. Web. 20 Nov. 2019
Mohammad, Mohammad, Elkady, Ehab, AND Fouad, Marwa. "Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin" European Journal of Chemistry [Online], Volume 3 Number 2 (30 June 2012)

DOI Link: https://doi.org/10.5155/eurjchem.3.2.152-155.570

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