European Journal of Chemistry

Development and validation of a reversed-phase column liquid chromatographic method for simultaneous determination of two novel gliptins in their binary mixtures with Metformin

Main Article Content

Mohammad Abdul-Azim Mohammad
Ehab Farouk Elkady
Marwa Ahmed Fouad


A new, simple, accurate, and precise liquid chromatographic method has been developed and validated for the determination of two novel dipeptidylpeptidase-4 (DPP-4) inhibitors; namely vildagliptin (VLG) and saxagliptin HCl (SXG) simultaneously in their binary mixtures with metformin HCl (MET). Chromatographic separation was achieved on an Inertsil® CN-3 column (250 mm x 4.6 mm, 5 µm). Isocratic elution using a mobile phase of potassium dihydrogen phosphate buffer pH (4.6) - acetonitrile (15:85, v:v) at a flow rate of 1 mL/min with UV detection at 208 nm was performed. The liquid chromatographic method was used for the simultaneous determination of either VLG, SXG and MET in the range of 5-200, 0.5-20 and 50-2000 μg/mL, respectively. The methods developed were satisfactorily applied to the analysis of the pharmaceutical formulations and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms.


icon graph This Abstract was viewed 3485 times | icon graph Article PDF downloaded 1159 times

How to Cite
Mohammad, M. A.-A.; Elkady, E. F.; Fouad, M. A. Development and Validation of a Reversed-Phase Column Liquid Chromatographic Method for Simultaneous Determination of Two Novel Gliptins in Their Binary Mixtures With Metformin. Eur. J. Chem. 2012, 3, 152-155.

Article Details

Crossref - Scopus - Google - European PMC

[1]. Singh, S. K.; Manne, N.; Pal, M. Beilstein J. Org. Chem. 2008, 4, 20-20.
PMid:18941490 PMCid:2486489

[2]. Nirogi, R.; Kandikere, V.; Mudigonda, K.; Komarneni, P.; Aleti, R.; Boggavarapu, R. Biomed. Chromatog. 2008, 22, 214-222.

[3]. El-Bagary, R. I.; Elkady, E. F.; Ayoub, B. M. Int. J. Biomed. Sci. 2011, 7(1), 55-61.

[4]. El-Bagary, R. I.; Elkady, E. F.; Ayoub, B. M. Int. J. Biomed. Sci. 2011, 7(3), 201-208.

[5]. Augeri, D.; Robl, J.; Betebenner, D.; Magnin, D.; Khanna, A.; Robertson, J. J. Med. Chem. 2005, 48, 5025-5037.

[6]. Kulasa, K.; Edelman, S. Core Evid. 2010, 5, 23-37.
PMid:21042540 PMCid:2963920

[7]. Fura, A.; Khanna, A.; Vyas, V.; Koplowitz, B.; Chang, S.; Caporuscio, C. Drug Metab. Disp. 2009, 37, 1164-1171.

[8]. Kalaichelvi, R.; Jayachandran, E. Int. J. Pharm. Pharm. Sci. 2011, 3, 179-180.

[9]. Sengupta, P.; Bhaumik, U.; Ghosh, A.; Sarkar, A.; Chatterjee, B.; Bose, A.; Pal, T. Chromatographia 2009, 69, 1243-1250.

[10]. Xiong, Y.; Zhou, H. J.; Zhang, Z. J. Chinese J. Anal. Lab. (Fenxi-Shiyanshi) 2006, 25(3), 5-8.

[11]. Ali, A. R.; Duraidi, I. I.; Saket, M.M.; Abu-Nameh, E. S. J. Aoac. Int. 2009, 92, 119-124.

[12]. Porta, V.; Schramm, S.; Kano, E.; Koono, E.; Armando, Y.; Fukuda, K; Serra, C. H. J. Pharm. Biomed. Anal. 2008, 46, 143-147.

[13]. El‐Bagary, R. I.; Elkady, E. F.; Ayoub, B. M. Talanta 2011, 85, 673-680.

[14]. Inturi, S.; Inturi, R.; Tagaram, I. K. Int. J. Pharm. Res. Dev. 2011, 3, 45-52.

[15]. Dongre, V. G.; Shah, S. B.; Karmuse, P. P.; Phadke, M.; Jadhav, V. K. J. Pharm. Biomed. Anal. 2008, 46, 583-586.

[16]. Lövgren, U.; Johansson, S.; Jensen, L. S.; Ekström, C.; Carlshaf, A. J. Pharm. Biomed. Anal. 2010, 53, 537-545.

[17]. United State Pharmacopoeia. USP 30, NF25, Rockville, MD, 2007.

[18]. The Indian Pharmacopoeia. 4th ed. New Delhi, The Controller of Publications; 1996.

Supporting Agencies

Most read articles by the same author(s)

Most read articles by the same author(s)


Dimensions - Altmetric - scite_ - PlumX

Downloads and views


Download data is not yet available.


Metrics Loading ...
License Terms

License Terms


Copyright © 2024 by Authors. This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License ( By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution, or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License ( are administered by Atlanta Publishing House LLC (European Journal of Chemistry).