European Journal of Chemistry 2016, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421

Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity


Nehal Fayek Farid (1) , Ibrahim Ahmed Naguib (2) , Radwa Saeed Moatamed (3) , Mohamed Refaat El-Ghobashy (4,*)

(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(3) National Organization for Drug Control and Research (NODCAR), Giza, 11843, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt
(*) Corresponding Author

Received: 03 Mar 2016, Accepted: 02 Apr 2016, Published: 30 Jun 2016

Abstract


A new sensitive, simple, rapid, accurate and precise spectrofluorimetric method for determination of diflunisal and its impurity is developed. Determination of diflunisal is based on first derivative spectrofluorimetric method, while its impurity can be determined by zero order spectrofluorimetric method. Diflunisal was measured at zero-crossing wavelength 394nm (zero crossing point with its impurity) which was selected for quantification of diflunisal. The impurity was measured directly at 334 nm, using 0.05 M phosphate buffer (pH = 9) as solvent. The analytical signal resulting from first derivative and zero order spectra were measured for diflunisal and its impurity, respectively. Linearity was over the range of 0.1-0.9 μg/mL for both with detection limit of 0.02 and 0.03 μg/mL and quantitation limit of 0.07 and 0.09 μg/mL for diflunisal and its impurity, respectively. The proposed method was validated as per ICH guidelines.The accuracy was checked by applying the proposed method for the determination of the drug and its impurity, the mean percentage recoveries were found to be 99.61±0.911 and 100.41±1.373 for diflunisal and its impurity, respectively. RSD values for repeatability testing were 0.268 and 0.569 and for intermediate precision were 0.224 and 0.259 for diflunisal and its impurity, respectively. The proposed method was effectively applied to analysis of studied drug in its tablet formulation. The results obtained by it were statistically compared with the reported method revealing high accuracy and good precision.


Keywords


DIF; BPL; Diflunisal; Biphenyl-4-ol; Zero order spectroflourimetry; First derivative spectrofluorimetry

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DOI: 10.5155/eurjchem.7.2.201-205.1421

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[1]. Nehal Fayek Farid, Nada Sayed Abdelwahab
Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance
European Journal of Chemistry  7(3), 329, 2016
DOI: 10.5155/eurjchem.7.3.329-333.1463
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How to cite


Farid, N.; Naguib, I.; Moatamed, R.; El-Ghobashy, M. Eur. J. Chem. 2016, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, N.; Naguib, I.; Moatamed, R.; El-Ghobashy, M. Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity. Eur. J. Chem. 2016, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, N., Naguib, I., Moatamed, R., & El-Ghobashy, M. (2016). Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity. European Journal of Chemistry, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, Nehal, Ibrahim Ahmed Naguib, Radwa Saeed Moatamed, & Mohamed Refaat El-Ghobashy. "Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity." European Journal of Chemistry [Online], 7.2 (2016): 201-205. Web. 19 Sep. 2019
Farid, Nehal, Naguib, Ibrahim, Moatamed, Radwa, AND El-Ghobashy, Mohamed. "Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity" European Journal of Chemistry [Online], Volume 7 Number 2 (30 June 2016)

DOI Link: https://doi.org/10.5155/eurjchem.7.2.201-205.1421

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