European Journal of Chemistry 2016, 7(2), 201-205 | doi: https://doi.org/10.5155/eurjchem.7.2.201-205.1421 | Get rights and content






  OPEN ACCESS | PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS

Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity


Nehal Fayek Farid (1) , Ibrahim Ahmed Naguib (2) , Radwa Saeed Moatamed (3) , Mohamed Refaat El-Ghobashy (4,*)

(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(3) National Organization for Drug Control and Research (NODCAR), Giza, 11843, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt
(*) Corresponding Author

Received: 03 Mar 2016 | Accepted: 02 Apr 2016 | Published: 30 Jun 2016 | Issue Date: June 2016

Abstract


A new sensitive, simple, rapid, accurate and precise spectrofluorimetric method for determination of diflunisal and its impurity is developed. Determination of diflunisal is based on first derivative spectrofluorimetric method, while its impurity can be determined by zero order spectrofluorimetric method. Diflunisal was measured at zero-crossing wavelength 394nm (zero crossing point with its impurity) which was selected for quantification of diflunisal. The impurity was measured directly at 334 nm, using 0.05 M phosphate buffer (pH = 9) as solvent. The analytical signal resulting from first derivative and zero order spectra were measured for diflunisal and its impurity, respectively. Linearity was over the range of 0.1-0.9 μg/mL for both with detection limit of 0.02 and 0.03 μg/mL and quantitation limit of 0.07 and 0.09 μg/mL for diflunisal and its impurity, respectively. The proposed method was validated as per ICH guidelines.The accuracy was checked by applying the proposed method for the determination of the drug and its impurity, the mean percentage recoveries were found to be 99.61±0.911 and 100.41±1.373 for diflunisal and its impurity, respectively. RSD values for repeatability testing were 0.268 and 0.569 and for intermediate precision were 0.224 and 0.259 for diflunisal and its impurity, respectively. The proposed method was effectively applied to analysis of studied drug in its tablet formulation. The results obtained by it were statistically compared with the reported method revealing high accuracy and good precision.


Keywords


DIF; BPL; Diflunisal; Biphenyl-4-ol; Zero order spectroflourimetry; First derivative spectrofluorimetry

Full Text:

PDF /    /


DOI: 10.5155/eurjchem.7.2.201-205.1421

Links for Article


| | | | |

| | | | | | |

Related Articles




Article Metrics

This Abstract was viewed 533 times | PDF Article downloaded 200 times

Funding information


Rameda Pharmaceutical Company, Egypt

Citations

/


[1]. Nehal Fayek Farid, Nada Sayed Abdelwahab
Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance
European Journal of Chemistry  7(3), 329, 2016
DOI: 10.5155/eurjchem.7.3.329-333.1463
/


References

[1]. The British Pharmacopoeia. Her Majesty's Stationery Office, London, 2010, pp. 2606-2610, 2615.

[2]. Maher, H. M. J. Liq. Chrom. Tech. 2014, 37, 634-652.
http://dx.doi.org/10.1080/10826076.2012.758134

[3]. Shaalan, R. A.; Belal, T. S. Sci. Pharm. 2013, 81, 713-731.
http://dx.doi.org/10.3797/scipharm.1301-24

[4]. Wahbi, A.; Mabrouk, M. M.; Moneeb, M. S. Pak. J. Pharm. Sci. 2009, 22, 8-17.

[5]. El-Saharty, Y.; Refaat, M.; El-Khateeb, S. Bull. Fac. Pharm. Cairo Univ. 2002, 40, 47-64.

[6]. Streete, P. Biomed. Sci. Appl. 1989, 495, 179-193.
http://dx.doi.org/10.1016/S0378-4347(00)82620-1

[7]. Bebawy, L; Elkousy, N. Pharm. Biomed. Anal. 1999, 20, 663-670.
http://dx.doi.org/10.1016/S0731-7085(99)00039-4

[8]. Haj, B. M; El-Arini, A. M.; Hassan, M. H.; Khadem, R. K.; Marzouq, M. S. Foren. Sci. Inter. 1999, 105, 141-53.
http://dx.doi.org/10.1016/S0379-0738(99)00106-1

[9]. Srinivas, B; Yadagiriswamy, P.; Venkateswarlu, G. Inter. J. Pharm. Sci. Res. 2015, 6, 1002-1010.

[10]. Sayin, F.; Kir, S. FABAD J. Pharm. Sci. 2004, 29, 121-126.

[11]. Murillo-Pulgarin, J. A; Alanon-Molina, A. Appl. Spectrosc. 2010, 64, 949-55.
http://dx.doi.org/10.1366/000370210792081055

[12]. Ioannou, P. C.; Lianidou, E. S.; Konstantianos, D. G. Anal. Chim. Acta 1995, 300, 237-241.
http://dx.doi.org/10.1016/0003-2670(94)00415-I

[13]. Pulgarin, M.; Molina, A.; Lopez, F. P. Anal. Chim. Acta 2007, 583, 55-62.
http://dx.doi.org/10.1016/j.aca.2006.10.009

[14]. Neeli, S. A.; Anuganti, H.; Sathesh, R.; Subrahmanyam, V. S. Dhaka Univ. J. Pharm. Sci. 2013, 12(1), 35-40.

[15]. Murillo, J.; Garcia, L. Anal. Lett. 1996, 29, 423-438.
http://dx.doi.org/10.1080/00032719608000408

[16]. Dress, J. C.; Wu, A. H. B. Analytical Techniques, Wolters Kluwer Vitalstream, 2008, Chapter 5, pp. 140.

[17]. ICH, Q. R., Validation of Analytical Procedures: Text and Methodology, ICH Harmonized Tripartite Guideline, 2005.

How to cite


Farid, N.; Naguib, I.; Moatamed, R.; El-Ghobashy, M. Eur. J. Chem. 2016, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, N.; Naguib, I.; Moatamed, R.; El-Ghobashy, M. Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity. Eur. J. Chem. 2016, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, N., Naguib, I., Moatamed, R., & El-Ghobashy, M. (2016). Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity. European Journal of Chemistry, 7(2), 201-205. doi:10.5155/eurjchem.7.2.201-205.1421
Farid, Nehal, Ibrahim Ahmed Naguib, Radwa Saeed Moatamed, & Mohamed Refaat El-Ghobashy. "Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity." European Journal of Chemistry [Online], 7.2 (2016): 201-205. Web. 27 Jan. 2020
Farid, Nehal, Naguib, Ibrahim, Moatamed, Radwa, AND El-Ghobashy, Mohamed. "Development and validation of spectrofluorimetric method for determination of diflunisal and its impurity" European Journal of Chemistry [Online], Volume 7 Number 2 (30 June 2016)

DOI Link: https://doi.org/10.5155/eurjchem.7.2.201-205.1421

| | | | |

| | | | | |

Save to Zotero Save to Mendeley

European Journal of Chemistry 2016, 7(2), 201-205 | doi: https://doi.org/10.5155/eurjchem.7.2.201-205.1421 | Get rights and content

Refbacks

  • There are currently no refbacks.




Copyright (c)




© Copyright 2019  Atlanta Publishing House LLC All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2019 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 4614 Lavista road, Tucker, GA, 30084, USA. Registered in USA.

hatay escort hatay escort corlu escort corum escort burgaz escort giresun escort aydin escort ordu escort erzincan escort hatay escort sivas escort rize escort edirne escort aksaray escort kibris escort isparta escort erzurum escort tekirdag escort usak escort urfa escort kastamonu escort kibris escort manisa escort giresun escort urfa escort nevsehir escort sivas escort yalova escort ordu escort hatay escort yalova escort amasya escort kayseri escort ordu escort maras escort canakkale escort yalova escort balikesir escort manisa escort urfa escort mugla escort trabzon escort bolu escort corlu escort diyarbakir escort isparta escort kutahya escort elazig escort erzurum escort sakarya escort afyon escort kutahya escort konya escort agri escort cesme escort sinop escort sivas escort konya escort kibris escort adapazari escort luleburgaz escort adana escort kibris escort rize escort sakarya escort alanya escort isparta escort burdur escort konya escort bitlis escort canakkale escort sivas escort amasya escort mus escort aydin escort van escort yalova escort kastamonu escort mardin escort bolu escort afyon escort sakarya escort isparta escort tokat escort trakya escort bayburt escort urfa escort mardin escort