European Journal of Chemistry 2016, 7(3), 329-333. doi:10.5155/eurjchem.7.3.329-333.1463

Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance


Nehal Fayek Farid (1,*) , Nada Sayed Abdelwahab (2)

(1) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(2) Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, 62514, Egypt
(*) Corresponding Author

Received: 07 Jun 2016, Accepted: 17 Jul 2016, Published: 30 Sep 2016

Abstract


Nowadays, analytical chemistry society is aware of the hazardous influence of chemicals on both health and environment. Additionally, chemists are more interested in eco-friendly methods of analysis. Levetiracetam (LEV) is an antiepileptic drug with no significant interaction with other medications and so it is a safe treatment in elderly people. The toxic pyridine-2-ol is reported in British Pharmacopoeia to be LEV impurity C (IMP-C) and related substance. A highly sensitive eco-friendly UPLC method was introduced for the first time for analysis of LEV and its toxic IMP. Separation has been carried out on CN column using 0.1% aqueous sodium lauryl sulphate:acetonitrile (7:93, v:v) with UV scanning at 205 nm. USP recommendations for method validation have been followed with respect to linearity, accuracy, robustness and ruggedness. The developed method was successfully applied for quantitation of LEV in its tablets dosage form and statistical analysis with the reported method showed no significant difference at confidence limit of 95%. The short run time (<3 minutes) and high sensitivity are of the most advantageous of developed method over the reported one.


Keywords


UPLC; Impurity; Validation; Eco-friendly; Pyridine-2-ol; Levetiracetam

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DOI: 10.5155/eurjchem.7.3.329-333.1463

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Citations

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[1]. Hazim M. Ali, Mohammed Gamal, Nada S. Abdelwahab, Nehal F. Farid
Simple GC–MS method for analysis of Levetiracetam and process-related toxic impurity
Microchemical Journal  146, 1236, 2019
DOI: 10.1016/j.microc.2019.02.063
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[2]. Nada S. Abdelwahab, Nehal F. Farid, Mohamed Elagawany, Esraa H. Abdelmomen
Efficient UPLC and CE methods for the simultaneous determination of azelastine hydrochloride and its genotoxic impurity
Biomedical Chromatography  32(11), e4346, 2018
DOI: 10.1002/bmc.4346
/


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How to cite


Farid, N.; Abdelwahab, N. Eur. J. Chem. 2016, 7(3), 329-333. doi:10.5155/eurjchem.7.3.329-333.1463
Farid, N.; Abdelwahab, N. Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance. Eur. J. Chem. 2016, 7(3), 329-333. doi:10.5155/eurjchem.7.3.329-333.1463
Farid, N., & Abdelwahab, N. (2016). Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance. European Journal of Chemistry, 7(3), 329-333. doi:10.5155/eurjchem.7.3.329-333.1463
Farid, Nehal, & Nada Sayed Abdelwahab. "Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance." European Journal of Chemistry [Online], 7.3 (2016): 329-333. Web. 22 Sep. 2019
Farid, Nehal, AND Abdelwahab, Nada. "Eco-friendly UPLC method for determination of Levetiracetam and its toxic related substance" European Journal of Chemistry [Online], Volume 7 Number 3 (30 September 2016)

DOI Link: https://doi.org/10.5155/eurjchem.7.3.329-333.1463

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