European Journal of Chemistry 2021, 12(3), 265-272 | doi: https://doi.org/10.5155/eurjchem.12.3.265-272.2093 | Get rights and content

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Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation


Mona Kamel Ahmed (1,*) orcid , Adel Magdy Michael (2) orcid , Said Abdel-Monem Hassan (3) orcid , Samah Sayed Abbas (4) orcid

(1) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ahram Canadian, Cairo, 11865, Egypt
(2) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ahram Canadian, Cairo, 11865, Egypt
(3) Department of Analytical Chemistry, Faculty of Pharmacy, University of Cairo, Cairo, ET-11562, Egypt
(4) Department of Analytical Chemistry, Faculty of Pharmacy, University of Cairo, Cairo, ET-11562, Egypt
(*) Corresponding Author

Received: 30 Jan 2021 | Revised: 18 Apr 2021 | Accepted: 04 May 2021 | Published: 30 Sep 2021 | Issue Date: September 2021

Abstract


Simple and precise spectrophotometric methods for quantitative assay of a mixture of hydrocortisone acetate (HCA) and clioquinol (CL) were developed and validated through different mathematical manipulation pathways. The developed methods utilized ratio spectra for resolving binary mixtures including absorbance subtraction, ratio subtraction coupled with spectrum subtraction, constant multiplication, constant value, and derivative ratio. The proposed methods were proved to be specific by analysing the laboratory-prepared mixtures and were applied for the assay of topical preparation successfully. The methods were validated using ICH guidelines where accuracy, repeatability and intermediate precision were within the acceptable limits. The linearity range was found to be 2-22 for HCA and 1.5-7 µg/mL for CL in all proposed methods and 2-7 µg/mL for HCA and CL in absorbance subtraction method through using a unified regression equation. The findings were statistically evaluated with respect to the official and reported methods, demonstrating that there was no significant difference.


Keywords


Clioquinol; Constant value; Derivative ratio; Ratio subtraction; Hydrocortisone acetate; Absorbance subtraction

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DOI: 10.5155/eurjchem.12.3.265-272.2093

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Funding information


October Pharma Company and Kahira Pharmaceutical Company, Egypt.

Citations

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[1]. Said A. Hassan, Nancy W. Nashat, Mohamed R. Elghobashy, Samah S. Abbas, Azza A. Moustafa
Advanced chemometric methods as powerful tools for impurity profiling of drug substances and drug products: Application on bisoprolol and perindopril binary mixture
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy  , 120576, 2021
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How to cite


Ahmed, M.; Michael, A.; Hassan, S.; Abbas, S. Eur. J. Chem. 2021, 12(3), 265-272. doi:10.5155/eurjchem.12.3.265-272.2093
Ahmed, M.; Michael, A.; Hassan, S.; Abbas, S. Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation. Eur. J. Chem. 2021, 12(3), 265-272. doi:10.5155/eurjchem.12.3.265-272.2093
Ahmed, M., Michael, A., Hassan, S., & Abbas, S. (2021). Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation. European Journal of Chemistry, 12(3), 265-272. doi:10.5155/eurjchem.12.3.265-272.2093
Ahmed, Mona, Adel Magdy Michael, Said Abdel-Monem Hassan, & Samah Sayed Abbas. "Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation." European Journal of Chemistry [Online], 12.3 (2021): 265-272. Web. 28 Nov. 2021
Ahmed, Mona, Michael, Adel, Hassan, Said, AND Abbas, Samah. "Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation" European Journal of Chemistry [Online], Volume 12 Number 3 (30 September 2021)

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