European Journal of Chemistry 2019, 10(3), 218-223 | doi: https://doi.org/10.5155/eurjchem.10.3.218-223.1880 | Get rights and content

Issue cover




Crossmark

  Open Access OPEN ACCESS | Open Access PEER-REVIEWED | RESEARCH ARTICLE | DOWNLOAD PDF | VIEW FULL-TEXT PDF | TOTAL VIEWS

Optimization and validation of a new chromatographic method for the assay of veterinary formulation


Hany Hunter Monir (1) orcid , Adel Magdy Michael (2) orcid , Christine Kamal Nessim (3,*) orcid , Yasmin Mohamed Fayez (4) orcid , Nahla Salah Elshater (5) orcid

(1) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, 11562, Cairo, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 12566, 6th of October City, Egypt
(3) Analytical Chemistry Department, Faculty of Pharmacy, Ahram Canadian University, 12566, 6th of October City, Egypt
(4) Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, 11562, Cairo, Egypt
(5) Senior Researcher Poultry Disease Reference Laboratory, Veterinary Quality Control on Poultry Production, 12211, Cairo, Egypt
(*) Corresponding Author

Received: 14 Apr 2019 | Revised: 03 Jun 2019 | Accepted: 14 Jun 2019 | Published: 30 Sep 2019 | Issue Date: September 2019

Abstract


New, validated and accurate reversed phase HPLC method with UV detection has been established for simultaneous determination of a veterinary binary mixture of doxycycline hydrochloride (DOX) and tylosin tartrate (TYT). The stationary phase was ACE- 126-2546 AQ C-18 (250 × 4.6 mm i.d., 5 μm particle size) column at 25 °C, in an isocratic mode, using mobile phase containing a mixture of methanol: acetonitrile: distilled water in the ratio of 60:20:20 (v:v:v), with 0.01% trichloroacetic acid at the flow rate of 0.8 mL/min and UV detection was performed at 270 nm. The retention times were 4.02±0.01 and 5.62±0.01 mins for DOX and TYT, respectively. Selective determination of the cited veterinary drugs has been developed in their formulation. The method was found to be linear over 1-50 µg/mL for DOX and TYT with mean percentage recoveries 99.62±1.220 and 100.09±1.104%. The method was proven to be accurate, precise and specific. The obtained results were statistically compared with those of the official and reported methods; using Student’s t test, F test and one-way ANOVA, showing no significant difference with high accuracy. Specificity of the applied method was assessed by analysing the laboratory-prepared mixtures and their combined dosage form. The developed method was confirmed according to ICH guidelines. The validated method can be considered as alternative and basic method for the routine determination of this fixed dose combination with minimum sample preparation.


Announcements


One of our sponsors will cover the article processing fee for all submissions made between May 17, 2023 and June 16, 2023 (Voucher code: SPONSOR2023).

Editor-in-Chief
European Journal of Chemistry

Keywords


UV detection; Tylosin tartrate; Veterinary binary mixture; Doxycycline hydrochloride; Validated RP-HPLC method; Simultaneous determination

Full Text:

PDF
PDF    Open Access

DOI: 10.5155/eurjchem.10.3.218-223.1880

Links for Article


| | | | | | |

| | | | | | |

| | | |

Related Articles




Article Metrics

icon graph This Abstract was viewed 1932 times | icon graph PDF Article downloaded 493 times

Funding information


Pharma Swede Veterinary Company, Egypt.

Citations

/


[1]. Adel M. Michael, Hayam M. Lotfy, Christine K. Nessim
Greenness profile and whiteness assessment of the stability-indicating HPLC method for the assay of levetiracetam
Microchemical Journal  190, 108669, 2023
DOI: 10.1016/j.microc.2023.108669
/

References


[1]. University of Michigan Health System Peptic Ulcer Disease. Accessed June 01, 2019. at: http://www.med.umich.edu/1info/FHP/ practiceguides/newpud/pud.pdf

[2]. British Pharmacopoeia, Her Majesty's Stationery Office, London, vol. I & II (P. 552) and vol. V (P. 6), 2013.

[3]. Sweetman, S. C., Martindale, The complete drug reference, 33rd ed. Pharmaceutical Press, London, 2002.

[4]. Watanabe, W.; Harter, T.; Bergamaschi, B. J. Environ. Quality 2008, 37, 78-85.
https://doi.org/10.2134/jeq2007.0371

[5]. Sun, P.; Barmaz, D.; Cabrera, M.; Pavlostathis and Huang, Ch. J. Chromatogr. A 2013, 1312, 10-17.
https://doi.org/10.1016/j.chroma.2013.08.044

[6]. Lotfy, H. M.; Maha, A. H.; Tawakkol, S.; Mohamed, E. H. Spectrochim. Acta A 2016, 153, 321-332.
https://doi.org/10.1016/j.saa.2015.07.106

[7]. Kogawa, A. C.; Salgado, H. R. N. World Res. J. Pharm. Res. 2013, 1(2), 021-024.

[8]. Lopez, P. J. L.; Martinez, C. J. J. Pharma. Biomed. Anal. 1993, 11, 1093-1098.

[9]. Salinas, F.; Berzas Nevado, J. J.; Espinosa, A. Analyst 1989, 114, 1141-1145.
https://doi.org/10.1039/an9891401141

[10]. Mahrous, M. S.; Abdel-Khalek, M. M. Talanta 1984, 31, 289-291.
https://doi.org/10.1016/0039-9140(84)80281-7

[11]. Attia, M. S.; Mahmoud, W. H.; Ramsis, M. N.; Khalil, L. H.; Othman, A. M.; Hashem, S. G.; Mostafa, M. J. Fluores. 2011, 21(4), 1739-1748.
https://doi.org/10.1007/s10895-011-0869-4

[12]. Van den Bogert, C.; Kroon, A. M. J. Pharm. Sci. 1981, 70, 186-189.
https://doi.org/10.1002/jps.2600700218

[13]. Salinas, F.; Pena, A. M.; Duran, M. I. Anal. Lett. 1990, 23, 863-876.
https://doi.org/10.1080/00032719008052487

[14]. Croubels, S.; Vermeersch, H.; Backeer, P.; Santos, M. D. F.; Remon, J. P.; Van Peteyhem, C. J. Chromatogr. B 1998, 708, 145-152.
https://doi.org/10.1016/S0378-4347(97)00644-0

[15]. Klimes, J. D. M. J. Chromatogr. A 1999, 846, 181-184.
https://doi.org/10.1016/S0021-9673(99)00148-X

[16]. Gajda, A.; Posyniak, A.; Pietruszka, K. Bull. Vet. Inst. Pulawy. 2008, 52, 417-420.

[17]. Gajda, A.; Posyniak, A.; Zmudzki, J.; Tomczyk, G. J. Chromatogr. B 2013, 928, 113-120.
https://doi.org/10.1016/j.jchromb.2013.03.011

[18]. Pavagada, J. R.; Kanakapura, B.; Kalsang, T.; Kanakapura, B. V.; Hosakere, D. R. J. Pre-Clin. Clin. Res. 2010, 4(2), 101-107.

[19]. Ruz, N.; Zabala, M.; Kramer, M. G.; Campanero, M. A.; Dios-Vieitez, M. C.; Blanco-Prieto, M. J. J. Chromatogr. 2004, 1031, 295-301.
https://doi.org/10.1016/j.chroma.2003.12.028

[20]. Cinquina, A. L.; Longo, F.; Anastasi, G.; Giannetti, L.; Cozzani, R. J. Chromatogr. A 2003, 987, 227-33.
https://doi.org/10.1016/S0021-9673(02)01446-2

[21]. Gastearena, I.; Dios-Vieitez, M. C.; Segura, E.; Goni, M. M.; Renedo, M. J.; Fos, D. Chromatographia 1993, 35, 524-526.
https://doi.org/10.1007/BF02267911

[22]. Zarghi, A.; Kebriaeezadeh, A.; Ahmadkhaniha, R. Bull. Chim. Farm. 2001, 140, 112-114.

[23]. Shoukry, A. F.; Badawy, S. S. Microchem. J. 1987, 36, 107-112.
https://doi.org/10.1016/0026-265X(87)90142-1

[24]. Issa, Y. M.; Abdel-Fattah, H. M.; Abdel-Moniem, N. B. Int. J. Electrochem. Sci. 2013, 8, 9578-9592.

[25]. Vargas, A., BSc Thesis, Faculty of LʼEcole Nationale Supérieure des Industries Chimiques and Worcester Polytechnic Institute, Nancy, France, 1-88, 2011.

[26]. Ghanem, M.; Saleh, A. J. Appl. Pharm. Sci. 2015, 5(1), 094-098.

[27]. Pietron, W.; Cybulski, W.; Kos, K.; Mitura, A. Bull. Vet. Inst. Pulawy 2011, 55, 725-729.

[28]. Ahmed, M. B. M.; Sree, Y. H. A.; Abdel-Fattah, S. M.; Hassan, N. S.; Saad, M. M. E. J. Planar Chromat. 2013, 26(5), 409-416.
https://doi.org/10.1556/JPC.26.2013.5.4

[29]. Kotha, S.; Sunitha, N.; Manoharbabu, S.; Inter. J. Pharm. Anal. Res. 2014, 3(2), 214-221.

[30]. Stan, G.; Badea, I. A.; Hassan, Y. A. J. Chromatogr. Sci. 2016, 54(9), 1567-1572.
https://doi.org/10.1093/chromsci/bmw105

[31]. United States Pharmacopoeia Commission, United States pharmacopeia-National formulary. United States Pharmacopoeial Inc., 2280-2282, 2004.

[32]. International Conference on Harmonization (ICH), Q2 (R1): Validation of Analytical Procedures: Text and Methodology, US FDA, Federal Register, ICH, 2005.

[33]. Lotfy, H. M.; Fayez, Y. M.; Michael, A. M.; Nessim, C. K. Spectrochim. Acta A 2016, 155, 11-20.
https://doi.org/10.1016/j.saa.2015.10.033

[34]. Hegazy, M. A.; Lotfy, H. M.; Rezk, M. R.; Omran, Y. R. Spectrochim. Acta A 2015, 140, 600-613.
https://doi.org/10.1016/j.saa.2014.12.098

[35]. Michael, A. M.; Shalliker, R. A. Ana


How to cite


Monir, H.; Michael, A.; Nessim, C.; Fayez, Y.; Elshater, N. Eur. J. Chem. 2019, 10(3), 218-223. doi:10.5155/eurjchem.10.3.218-223.1880
Monir, H.; Michael, A.; Nessim, C.; Fayez, Y.; Elshater, N. Optimization and validation of a new chromatographic method for the assay of veterinary formulation. Eur. J. Chem. 2019, 10(3), 218-223. doi:10.5155/eurjchem.10.3.218-223.1880
Monir, H., Michael, A., Nessim, C., Fayez, Y., & Elshater, N. (2019). Optimization and validation of a new chromatographic method for the assay of veterinary formulation. European Journal of Chemistry, 10(3), 218-223. doi:10.5155/eurjchem.10.3.218-223.1880
Monir, Hany, Adel Magdy Michael, Christine Kamal Nessim, Yasmin Mohamed Fayez, & Nahla Salah Elshater. "Optimization and validation of a new chromatographic method for the assay of veterinary formulation." European Journal of Chemistry [Online], 10.3 (2019): 218-223. Web. 3 Jun. 2023
Monir, Hany, Michael, Adel, Nessim, Christine, Fayez, Yasmin, AND Elshater, Nahla. "Optimization and validation of a new chromatographic method for the assay of veterinary formulation" European Journal of Chemistry [Online], Volume 10 Number 3 (30 September 2019)

The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item



DOI Link: https://doi.org/10.5155/eurjchem.10.3.218-223.1880


CrossRef | Scilit | GrowKudos | Researchgate | Publons | ScienceGate | Scite | Lens | OUCI

WorldCat Paperbuzz | LibKey Citeas | Dimensions | Semanticscholar | Plumx | Kopernio | Zotero | Mendeley

ZoteroSave to Zotero MendeleySave to Mendeley



European Journal of Chemistry 2019, 10(3), 218-223 | doi: https://doi.org/10.5155/eurjchem.10.3.218-223.1880 | Get rights and content

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Authors

Creative Commons License
This work is published and licensed by Atlanta Publishing House LLC, Atlanta, GA, USA. The full terms of this license are available at http://www.eurjchem.com/index.php/eurjchem/pages/view/terms and incorporate the Creative Commons Attribution-Non Commercial (CC BY NC) (International, v4.0) License (http://creativecommons.org/licenses/by-nc/4.0). By accessing the work, you hereby accept the Terms. This is an open access article distributed under the terms and conditions of the CC BY NC License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited without any further permission from Atlanta Publishing House LLC (European Journal of Chemistry). No use, distribution or reproduction is permitted which does not comply with these terms. Permissions for commercial use of this work beyond the scope of the License (http://www.eurjchem.com/index.php/eurjchem/pages/view/terms) are administered by Atlanta Publishing House LLC (European Journal of Chemistry).


© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.

The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.

Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.