Email this article 
Hide
Show all
OPEN ACCESS | 
PEER-REVIEWED |
RESEARCH ARTICLE
|
DOWNLOAD PDF
|
VIEW FULL-TEXT PDF
| TOTAL VIEWS
Simultaneous quantitative analysis of tamsulosin and finasteride in pharmaceutical dosage form by U-HPLC Tandem mass spectrometry
Dalia Mohamed (1,*)
, Shereen Mowaka (2) , Ahmed Mostafa (3) (1) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795, Cairo, Egypt
(2) Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795, Cairo, Egypt
(3) Pharmaceutical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795, Cairo, Egypt
(*) Corresponding Author
Received: 05 Oct 2013 | Revised: 02 Nov 2013 | Accepted: 02 Nov 2013 | Published: 31 Mar 2014 | Issue Date: March 2014
Abstract
A sensitive, rapid, selective and accurate liquid chromatography coupled to quadrupole tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous identification and quantification of tamsulosin and finasteride in bulk and in their combined dosage form. Chromatography was performed on a Hypersil gold 50 mm × 2 mm (1.9 μm) column, using acetonitrile:ammonium acetate (90:10, v:v) pH = 3.5 as the mobile phase. Protonated ions formed by a turbo ion-spray in positive mode were used to detect the analytes as well as the internal standard (IS). MS/MS detection was carried out by monitoring the fragmentation of 408.74 → 227.29 (m/z), 373.11 → 304.96 (m/z) and 255.75 → 166.15 (m/z) for tamsulosin, finasteride and diphenhydramine (IS), respectively, on a triple quadrupole mass spectrometer. The linearity was obtained over the concentration range of 1.6-40.0 ng/mL for tamsulosin and 20.0-500.0 ng/mL for finasteride with a lower limit of detection of 0.5 ng/mL and 5.0 ng/mL for the two drugs, respectively. The proposed method was successfully applied to tamsulosin and finasteride determination in pharmaceutical dosage form. The results obtained were statistically analyzed and compared with those of reference ones; in addition, the method was validated according to USP 34 recommendations. The simplicity and sensitivity of this method allows its use in the quality control of the cited drugs and can be extended for routine analysis of the drugs in their pharmaceutical preparations.
Announcements
Our editors have decided to support scientists to publish their manuscripts in European Journal of Chemistry without any financial constraints.
1- The article processing fee will not be charged from the articles containing the single-crystal structure characterization or a DFT study between September 15, 2023 and October 31, 2023 (Voucher code: FALL2023).
2. A 50% discount will be applied to the article processing fee for submissions made between September 15, 2023 and October 31, 2023 by authors who have at least one publication in the European Journal of Chemistry (Voucher code: AUTHOR-3-2023).
3. Young writers will not be charged for the article processing fee between September 15, 2023 and October 31, 2023 (Voucher code: YOUNG2023).
Editor-in-Chief
European Journal of Chemistry
Keywords
Full Text:
PDF
DOI: 10.5155/eurjchem.5.1.181-185.942
Links for Article
| | | | | | |
| | | | | | |
| | | |
Related Articles
Article Metrics
This Abstract was viewed 1607 times |
PDF Article downloaded 728 times
Citations
[1]. Ahmed Abdalazim Mostafa, Maha Mahmoud Abou-El Alamin
Novel approach for UPLC-ESI-Tandem mass spectrometric method for simultaneous determination of olmesartan medoxomil and hydrochlorothiazide in their pharmaceutical combination with kinetic studies of forced degradation
European Journal of Chemistry 7(3), 309, 2016
DOI: 10.5155/eurjchem.7.3.309-314.1450
[2]. Shereen A. Boltia, Mohammed Abdelkawy, Taghreed A. Mohamed, Nahla N. Mostafa
Optimized ion selective electrode for determination of Tamsulosin hydrochloride in the presence of co-formulated Deflazacort in bulk, tablet dosage form and human urine
Microchemical Journal 163, 105936, 2021
DOI: 10.1016/j.microc.2021.105936
References
[1]. Dunn, C. J.; Matheson, A.; Faulds, D. M. Drugs Aging 2002, 19, 135-161.
http://dx.doi.org/10.2165/00002512-200219020-00004
[2]. Narayan, P.; Rao, T. H. S. G. Rev. Urol. 2005, 7, 42-48.
[3]. Honda, K.; Nakagawa, C. J. Pharmacol. Exp. Ther. 1986, 239, 512-516.
[4]. Honda, K.; Nakagawa, C.; Terai, M.; Naunyn, S. Arch. Pharmacol. 1987, 336, 295-302.
http://dx.doi.org/10.1007/BF00172681
[5]. The United States Pharmacopeia 34, NF 29, USP Convention, Rockville, MD, USA, 2011.
[6]. Kumari, R.; Dash, P. P.; Lal, V. K.; Mishra, A.; Murthy, P. N. Indian J. Pharm. Sci. 2010, 72, 785-787.
http://dx.doi.org/10.4103/0250-474X.84596
[7]. Sudha, T.; Jitendra, D. Int. J. Chem. Res. 2011, 2, 29-33.
[8]. Chandorkar, J. G.; Kotwal, V. B.; Dhande, N. S.; Gurav, S. G.; Pande, V. V.; Yadav, P. V. Pak. J. Pharm. Sci. 2008, 21, 307-310.
[9]. Supriya, M. M.; Roshani, Y. V.; Suvarna, I. B. Int. J. Pharm. Pharm. Sci. 2012, 4, 319-322.
[10]. Rao, B. M.; Srinivasu, M. K.; Sridhar, G.; Reddy, B. S.; Vittal, T. V.; Kumar, R. P. Indian Drugs 2006, 43, 39-43.
[11]. Bari, S. B.; Bakshi, A. R.; Jain, P. S.; Surana, S. J. Chromatogr. Res. Int. 2011, 2011, 1-6
[12]. Nageswara, R. R.; Kumar, T. M. V.; Narasa, R. A.; Shinde, D. D.; Ramanjaneyulu, G. S. J. Pharm. Biomed. Anal. 2008, 46, 94-103.
http://dx.doi.org/10.1016/j.jpba.2007.09.009
[13]. Ramakrishna, N. V. S.; Vishwottan, K. N.; Manoj, S.; Koteshwara, M.; Wishu, S.; Varma, D. P. Biomed. Chromatogr. 2005, 19, 709-719.
http://dx.doi.org/10.1002/bmc.498
[14]. Keski-Rahkonen, P.; Parssinen, O.; Leppanen, E.; Mauriala, T.; Lehtonen, M.; Auriola, S. J. Pharm. Biomed. Anal. 2007, 43, 606-612.
http://dx.doi.org/10.1016/j.jpba.2006.07.016
[15]. Rao, B. M.; Srinivasu, M. K.; Thilakumar, T.; More, S.; Rajendra, K. P. Indian Drugs 2005, 42, 175-177.
[16]. Ozkan, S. A.; Uslu, B.; Aboul-Enein, H. Y. Talanta 2003, 61, 147-156.
http://dx.doi.org/10.1016/S0039-9140(03)00248-0
[17]. Maier, V.; Horakova, J.; Petr, J.; Tesarova, C.; Coufal, P.; Sevcik, J. J. Pharm. Biomed. Anal. 2005, 39, 691-696.
http://dx.doi.org/10.1016/j.jpba.2005.04.023
[18]. Patel, N. U.; Chaudhari, B. G. Pharm. Sin. 2011, 2, 172-175.
[19]. Gadhave, N. A.; Sawant, S. D.; Ghante, M. R.; Nikam, A. D. Int. J. Pharm. Res. Dev. 2011, 3, 87-92.
[20]. Saradhi, S. V.; Meherjaha, S. K.; Jyothsna, N.; Priyanka, A.; Sirisha, P. B.; Ramakrishna, C. H.; Sekaran, C. B. J. Pharm. Sci. Res. 2012, 4, 1958-1963.
[21]. Thompson, I. M.; Klein, E. A.; Lippman, S. M.; Coltman, C. A.; Djavan, B. Eur. Urol. 2003, 4, 650-655.
http://dx.doi.org/10.1016/j.eururo.2003.11.001
[22]. Syed, A. A.; Amshumali, M. K. J. Pharm. Biomed. Anal. 2001, 25, 1015-1019.
http://dx.doi.org/10.1016/S0731-7085(01)00385-5
[23]. Amer, S. M. Farmaco 2003, 58, 159-163.
http://dx.doi.org/10.1016/S0014-827X(02)00015-0
[24]. Matuszewski, B. K.; Constanzer, M. L.; Chavez-Eng, C. M. Anal. Chem. 1998, 70, 882-889.
http://dx.doi.org/10.1021/ac971078+
[25]. Mousavi, S. H. H.; Kobarfard, F.; Husain, S. W.; Tehrani, M. S.; Azar, P. A.; Ahmadkhaniha, R.; Mehdizadeh, A. Iran. J. Pharm. Res. 2012, 11, 59-67.
[26]. Thimmaraju, M. K.; Rao, V.; Gurrala, S. Pharm. Lett. 2011, 3, 79-86.
[27]. Patel, D. B.; Patel, N. J. Acta. Pharm. 2010, 60, 197-205.
[28]. Miller J. C.; Miller, J. N. Statistics for Analytical Chemistry, 5th edition, Wiley, New York, 2005, p. 256.
How to cite
The other citation formats (EndNote | Reference Manager | ProCite | BibTeX | RefWorks) for this article can be found online at: How to cite item
DOI Link: https://doi.org/10.5155/eurjchem.5.1.181-185.942
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
| 
Save to Zotero
Save to Mendeley
European Journal of Chemistry 2014, 5(1), 181-185 | doi: https://doi.org/10.5155/eurjchem.5.1.181-185.942 | Get rights and content
Refbacks
- There are currently no refbacks.
Copyright (c)
© Copyright 2010 - 2023 • Atlanta Publishing House LLC • All Right Reserved.
The opinions expressed in all articles published in European Journal of Chemistry are those of the specific author(s), and do not necessarily reflect the views of Atlanta Publishing House LLC, or European Journal of Chemistry, or any of its employees.
Copyright 2010-2023 Atlanta Publishing House LLC. All rights reserved. This site is owned and operated by Atlanta Publishing House LLC whose registered office is 2850 Smith Ridge Trce Peachtree Cor GA 30071-2636, USA. Registered in USA.